AGE &HORMONE DEPENDENT REGULATION OF HEPATIC PROTEIN

Project: Research project

Project Details

Description

The long-term goal of the proposed research is a better understanding of
the molecular mechanism of the aging process and its relationship to
changing endocrine environment. This aim will be pursued through the
regulatory studies on the age- and hormone-dependent synthesis of a hepatic
protein (SMP-2). SMP-2 has been identified and characterized as a
biomarker for hormone action and aging. The proposed research will
emphasize the following: 1) isolation and characterization of full length
SMP-2 cDNA clones for two isoforms of SMP-2 using an expression vector; 2)
isolation and characterization of the genomic clones for SMP-2; 3)
identification of nuclear nonhistone proteins that may show high affinity
for the regulatory region of SMP-2 gene; 4) identification of the SMP-2
synthesizing hepatocytes by the immunohistochemical method in order to
explore possible cellular specialization in the synthesis of SMP-2; 5)
producation of monoclonal antibodies to the both forms of SMP-2; 6)
elucidation of the mechanism of androgenic repression of SMP-2 and its mRNA
using such experiments as nuclear run off assays, and pulse-chase studies
for analysis of mRNA stability; 7) exploration of possible tissue diversity
in the synthesis of SMP-2 using RNA dot blot assay and
immunohistochemistry; and 8) investigation of the time course of
reappearance of SMP-2 at the old age, in caloric restricted rats, by
measuring the SMP-2 mRNA level.
StatusFinished
Effective start/end date9/1/824/30/98

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $162,216.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $177,600.00
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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