The Biomolecular NMR Core Facility offers access to three state-of-the-art high field spectrometers. Instruments in the NMR facility include a Bruker AVIII 500 MHz, a Bruker AVI 600 MHz, and a Bruker AVNeo 700 MHz. The spectrometers are each equipped with a variable temperature controller and high sensitivity cryogenically cooled 1H/13C/15N probes. Additionally, the 500 and 700 MHz NMR spectrometers are both equipped with automatic sample changers (SampleJet and SampleExpress, respectively). There is also a Gilson 513 liquid handler available for the automated preparation of samples.
The 500 MHz spectrometer is ideally equipped for determining the structures of natural products with anticancer activity and for carrying out NMR-based fragment screening. The small-volume, 1.7 mm cryoprobe requires only 40 uL of sample volume and delivers exceptional mass sensitivity. The 600 and 700 MHz spectrometers are ideally configured for determining structures of larger macromolecules and their complexes with ligands including bioactive small molecules, purified natural produces or peptides. NMR is exceptionally well suited for mapping interaction sites in macromolecular complexes and is exquisitely sensitive to relatively weak interactions that cannot be easily detected by other biophysical methods.
The facility also maintains a Jasco 815 Circular Dichroism Spectrometer equipped with a variable temperature controller. The primary use of this CD is analyzing the secondary structure of proteins and conformation of nucleic acids. CD-based determination of melting temperatures yields insight into stability of macromolecular structures.