Description
Accession Number: GSE18281
Platform:
GPL1261: [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Organism: Mus musculus
Published on 2009-11-24
Summary:
Interaction of hematopoietic progenitors with the thymic stromal microenvironment induces them to proliferate, adopt the T cell fate, and asymmetrically diverge into multiple T lineages. Progenitors at various developmental stages are stratified among different regions of the thymus, implying that the corresponding microenvironments differ from one another, and provide unique sets of signals to progenitors migrating between them. The nature of these differences remains undefined. Here we use novel physical and computational approaches to characterize these stromal subregions, distinguishing gene expression in microdissected tissues from that of their lymphoid constituents. Using this approach, we comprehensively map gene expression in functionally distinct stromal microenvironments, and identify clusters of genes that define each region. Quite unexpectedly, we find that the central cortex lacks distinctive features of its own, and instead appears to function by sequestering unique microenvironments found at the cortical extremities, and modulating the relative proximity of progenitors moving between them.
Overall Design:
4 to 6 weeks old male C57bl6/J were used for microdissection of 3 thymic cortical subregions and thymic medulla or for sorting cortical and medullary thymocytes. These samples were used for subsequent RNA purification, labeling and hybridization to Affymetrix arrays
Contact:
Name: howard t petrie
Organization: the scripps research instititute
Deparment: cancer biology
Address: 130 scripps way jupiter FL 33458 USA
Email: [email protected]
Phone: 561 228 3205
Organization: Affymetrix, Inc.
Address: Santa Clara CA 95051 USA
Email: [email protected], [email protected]
Phone: 888-362-2447
Web-Link: http://www.affymetrix.com/index.affx
Platform:
GPL1261: [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Organism: Mus musculus
Published on 2009-11-24
Summary:
Interaction of hematopoietic progenitors with the thymic stromal microenvironment induces them to proliferate, adopt the T cell fate, and asymmetrically diverge into multiple T lineages. Progenitors at various developmental stages are stratified among different regions of the thymus, implying that the corresponding microenvironments differ from one another, and provide unique sets of signals to progenitors migrating between them. The nature of these differences remains undefined. Here we use novel physical and computational approaches to characterize these stromal subregions, distinguishing gene expression in microdissected tissues from that of their lymphoid constituents. Using this approach, we comprehensively map gene expression in functionally distinct stromal microenvironments, and identify clusters of genes that define each region. Quite unexpectedly, we find that the central cortex lacks distinctive features of its own, and instead appears to function by sequestering unique microenvironments found at the cortical extremities, and modulating the relative proximity of progenitors moving between them.
Overall Design:
4 to 6 weeks old male C57bl6/J were used for microdissection of 3 thymic cortical subregions and thymic medulla or for sorting cortical and medullary thymocytes. These samples were used for subsequent RNA purification, labeling and hybridization to Affymetrix arrays
Contact:
Name: howard t petrie
Organization: the scripps research instititute
Deparment: cancer biology
Address: 130 scripps way jupiter FL 33458 USA
Email: [email protected]
Phone: 561 228 3205
Organization: Affymetrix, Inc.
Address: Santa Clara CA 95051 USA
Email: [email protected], [email protected]
Phone: 888-362-2447
Web-Link: http://www.affymetrix.com/index.affx
| Date made available | Sep 25 2009 |
|---|---|
| Publisher | Gene Expression Omnibus |