Additional file 7: Figure S6. of MicroRNA-195 acts as an anti-proliferative miRNA in human melanoma cells by targeting Prohibitin 1

  • Priscila Daniele Ramos Cirilo (Creator)
  • Luciana Nogueira de Sousa Andrade (Contributor)
  • Bruna R. Correa (Creator)
  • Mei Qiao (Creator)
  • Tatiane Katsue Furuya (Contributor)
  • Roger Chammas (Creator)
  • Luiz O Penalva (Contributor)



Drug-induced cell death is accentuated by miR-195. This panel shows the cell cycle profile of SK-MEL-5 melanoma cells transfected with either miRNA-control/miR-195 (10 nM) (24 h) and treated with cisplatin (CIS-2.5 and 5 μM) or temozolomide (TMZ-50 and 250 μM) for 48 h (total time 72 h). The percentage of the cell population distributed in each cell cycle phase is indicated: G0/G1 = blue, S = green, and G2/M = pink. (a)-MicroRNA-195 alone increased cell death (cells accumulated at sub G0/G1). (b-e) Treatment with drugs induces mainly arrest of SK-MEL-5 cells in G2/M whereas the cytotoxic effects of cisplatin and temozolomide were higher when combined with miR-195 transfection, inducing cell death (sub G0/G1 cells population). Cell cycle distribution of propidium iodide (PI)-labeled cells was analyzed using FlowJo Cytometric software. Representative examples of at least three independent experiments are reported. (JPEG 314 kb)
Date made available2017

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