Additional file 10: of Probabilistic modeling of personalized drug combinations from integrated chemical screen and molecular data in sarcoma

Figure S10. Schematic of PTIM-informed U23674 rewiring experiment. An initial culture of U23674 is screened using the Roche screen. The same culture is used to seed 6 new cultures, which are grown until the cell population is sufficient for drug screening. Five of the 6 cultures were treated using single agents and drug combinations in low dosages (75â nM OSI-906, 50â nM GDC-0941) and one culture was left untreated. After treatment and incubation for 72â h, the compounds were removed the cells were screened using the Roche Orphan Kinome screen. (TIF 8496 kb)