Additional file 1: Figure S1. of Mediator subunit Med12 contributes to the maintenance of neural stem cell identity

Unbiased genome-wide expression profiling analysis validation (A, C, D) RNA from mNS-5 NSCs infected with lentiviruses expressing non-specific (NS) control or Med12-specific shRNAs was harvested and subjected to RT-qPCR analysis. mRNA levels for each gene were normalized to β-actin mRNA and expressed relative to their corresponding mRNA levels in NS control shRNA-expressing cells. In (C, D) relative mRNA levels for 29 randomly selected genes from the original microarray-derived list were determined using the same RNA samples subjected to microarray analysis (C) as well as RNA from an independent set of knockdown experiments (D). Data represent the mean +/− SEM of at least three independent experiments performed in triplicate. Asterisks denote statistically significant differences relative to NS control shRNA (Student’s t-test, *p